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The Core Laboratory Facility at the Virginia Bioinformatics Institute at Virginia Tech has installed Ion Torrent’s new, state-of-the-art Personal Genome Machine™, adding revolutionary semiconductor sequencing technology to its cutting edge Next Generation Sequencing services.
Ion Torrent pairs semiconductor technology with a basic, well-characterized biochemical process to perform massively parallel sequencing without using the light-releasing reactions and sensitive cameras required for other sequencing platforms. This provides a direct link between chemical and digital data, allowing for a fast, scalable sequencing solution at an affordable price.
Acquisition of the PGM™ system strengthens the Core Laboratory’s reputation as a one-stop shop for all sequencing applications. Ion Torrent boasts one of the fastest workflows around, making it possible to fully prepare eight samples in less than six hours. The Personal Genome Machine™ is capable of producing more than 10 megabases of data with read lengths of up to 200 base pairs in a single two-hour sequencing run, allowing researchers to rapidly and inexpensively bridge the gap between hypotheses and results. Multiplex amplicon, transcriptome, and small RNA sequencing are ideal for the PGM™ sequencer, and it is fully compatible with applications such as paired-end sequencing, ChIP-Seq, and methylation. Ion Torrent service offerings in the Core Laboratory Facility place the VBI on the leading edge of the next wave of sequencing technology.
Applications:
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Small Genome Sequencing- Microbial and viral de novo & resequencing
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Targeted Resequencing
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Whole Genome/Exome Validation- validate other platform results
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RNA Sequencing- Whole Transcriptome
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ChIP-Seq
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Amplicon Sequencing
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Copy Number Anlaysis, Methylation, Paired Ends
Accepted Samples Types:
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Genomic DNA
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PCR Products
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BACs
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cDNA
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RNA
DNA sample requirements:
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5-10ug DNA
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Should not be degraded
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Include gel image of product
RNA sample Requirements:
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RNA should not be degraded
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RNA should have RNA integrity number (RIN) greater than 7
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Whole Transcriptome- 100-500 ng of poly(A) RNA or 200-500 ng of rRNA-depleted total RNA
Price Quotes and Planning:
Please contact iontorrent@vbi.vt.edu prior to sample submission for quotes and planning.
Expected Results:
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314 chip: starting at 10Mb of high quality sequence
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316 chip: starting at 100Mb of high quality sequence
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318 chip: starting at 1Gb of high quality sequence
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200bp read lengths, 400bp in 2012
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SFF/FASTQ file output
Data Analysis:
Please contact our Data Analysis Core at dac@vbi.vt.edu (540-231-2777) for quotes and planning.
Pricing for Ion Torrent:
Virginia Tech Researchers
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DNA/RNA Library Prep: $475 per sample
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Amplicon Library Prep: $275 per sample
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Data Generation (314): $343 per run
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Data Generation (316): Please enquire
Non-Virginia Tech Researchers
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DNA/RNA Library Prep: $758 per sample
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Amplicon Library Prep: $439 per sample
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Data Generation (314): $547 per run
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Data Generation (316): Please enquire
For More Information:
Please contact us at iontorrent@vbi.vt.edu (540-231-1229).
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