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Purwantini,
Endang
, Ph.D
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| Faculty
- Publications
- Purwantini, Endang
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Senior Research Associate
Phone: (540) 231-4395
Email:epurwant@vt.edu
Fax: 540-231-2606
Personal_Page
| Microbial Diversity Group
| Mycobacterial Research Program
Publications:
Hasan MR, Rahman M, Jaques S, Purwantini E, Daniels L. Glucose 6-phosphate accumulation in mycobacteria: implications for a novel F420-dependent anti-oxidant defense system. J Biol Chem. 2010;285:19135–19144. http://www.ncbi.nlm.nih.gov/pubmed/20075070
Purwantini E, Mukhopadhyay B. Conversion of NO2 to NO by reduced coenzyme F420 protects mycobacteria from nitrosative damage. Proc Natl Acad Sci U S A. 2009;106:6333–6338. http://www.ncbi.nlm.nih.gov/pubmed/19325122
Rajasekaran P, Seleem MN, Contreras A, et al. Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression. Appl Environ Microbiol. 2008;74:7051–7055. http://www.ncbi.nlm.nih.gov/pubmed/18836016
Lai H, Kraszewski JL, Purwantini E, Mukhopadhyay B. Identification of pyruvate carboxylase genes in Pseudomonas aeruginosa PAO1 and development of a P. aeruginosa-based overexpression system for alpha4- and alpha4beta4-type pyruvate carboxylases. Appl Environ Microbiol. 2006;72:7785–7792. http://www.ncbi.nlm.nih.gov/pubmed/16997990
Mukhopadhyay B, Purwantini E, Kreder CL, Wolfe RS. Oxaloacetate synthesis in the methanarchaeon Methanosarcina barkeri: pyruvate carboxylase genes and a putative Escherichia coli-type bifunctional biotin protein ligase gene (bpl/birA) exhibit a unique organization. J Bacteriol. 2001;183:3804–3810. http://www.ncbi.nlm.nih.gov/pubmed/11371547
Mukhopadhyay B, Purwantini E. Pyruvate carboxylase from Mycobacterium smegmatis: stabilization, rapid purification, molecular and biochemical characterization and regulation of the cellular level. Biochim Biophys Acta. 2000;1475:191–206. http://www.ncbi.nlm.nih.gov/pubmed/10913817
Purwantini E, Daniels L. Molecular analysis of the gene encoding F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis. J Bacteriol. 1998;180:2212–2219. http://www.ncbi.nlm.nih.gov/pubmed/9555906
Purwantini E, Gillis TP, Daniels L. Presence of F420-dependent glucose-6-phosphate dehydrogenase in Mycobacterium and Nocardia species, but absence from Streptomyces and Corynebacterium species and methanogenic Archaea. FEMS Microbiol Lett. 1997;146:129–134. http://www.ncbi.nlm.nih.gov/pubmed/8997717
Klein AR, Berk H, Purwantini E, Daniels L, Thauer RK. Si-face stereospecificity at C5 of coenzyme F420 for F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis and F420-dependent alcohol dehydrogenase from Methanoculleus thermophilicus. Eur J Biochem. 1996;239:93–97. http://www.ncbi.nlm.nih.gov/pubmed/8706724
Purwantini E, Daniels L. Purification of a novel coenzyme F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis. J Bacteriol. 1996;178:2861–2866. http://www.ncbi.nlm.nih.gov/pubmed/8631674
Mukhopadhyay B, Purwantini E, Pihl TD, Reeve JN, Daniels L. Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli. J Biol Chem. 1995;270:2827–2832. http://www.ncbi.nlm.nih.gov/pubmed/7852356
Purwantini E, Mukhopadhyay B, Daniels L. Characterization of F-390 Synthetase-Activity in Cell-Extracts of Methanobacterium-Thermoautotrophicum Marburg. Canadian Journal of Microbiology. 1994;40:306–309.
Mukhopadhyay B, Purwantini E, Daniels L. Effect of Methanogenic Substrates on Coenzyme F-420-Dependent N-5,N-10-Methylene-H-4 Mpt Dehydrogenase, N-5,N-10-Methenyl-H-4 Mpt Cyclohydrolase and F-420-Reducing Hydrogenase Activities in Methanosarcina-Barkeri. Archives of Microbiology. 1993;159:141–146.
Purwantini E, Mukhopadhyay B, Spencer RW, Daniels L. Effect of temperature on the spectral properties of coenzyme F420 and related compounds. Anal Biochem. 1992;205:342–350. http://www.ncbi.nlm.nih.gov/pubmed/1443583
Mukhopadhyay B, Purwantini E, Demacario EC, Daniels L. Characterization of a Methanosarcina Strain Isolated from Goat Feces, and That Grows on H2-Co2 Only after Adaptation. Current Microbiology. 1991;23:165–173.
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