© 2000 - 2012 Virginia Bioinformatics Institute
Wednesday, 16 May 2012
Hefta, Stanley , Ph.D.
Faculty - Publications - Hefta, Stanley

Director, Strategic Planning and Business Development

Phone: (540) 231-6585
Email:shefta@vbi.vt.edu
Fax: (540) 231-2606

Administrative Specialist: Erin Sexton
Phone: (540) 231-8138
Email: ecsexton@vbi.vt.edu
Fax:540-231-2606

Personal_Page | Strategic Business Development and Planning Group



Publications:

2008


Tao L, Kiefer SE, Xie D, Bryson JW, Hefta SA, Doyle ML. Time-resolved limited proteolysis of mitogen-activated protein kinase-activated protein kinase-2 determined by LC/MS only. J Am Soc Mass Spectrom. 2008;19(6):841–854.  http://www.ncbi.nlm.nih.gov/pubmed/18400511

2005


Gao J, Garulacan LA, Storm SM, et al. Biomarker discovery in biological fluids. Methods. 2005;35(3):291–302.  http://www.ncbi.nlm.nih.gov/pubmed/15722225

Omenn GS, States DJ, Adamski M, et al. Overview of the HUPO Plasma Proteome Project: results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database. Proteomics. 2005;5(13):3226–3245.  http://www.ncbi.nlm.nih.gov/pubmed/16104056

2004


Fach EM, Garulacan LA, Gao J, et al. In vitro biomarker discovery for atherosclerosis by proteomics. Mol Cell Proteomics. 2004;3(12):1200–1210.  http://www.ncbi.nlm.nih.gov/pubmed/15496433

Gao J, Ann Garulacan L, Storm SM, et al. Identification of in vitro protein biomarkers of idiosyncratic liver toxicity. Toxicol In Vitro. 2004;18(4):533–541.  http://www.ncbi.nlm.nih.gov/pubmed/15130611

Ho K Q. Xiao E. Fach J. Gao P. Kayne A.R. Dongre S.A. Hefta G. Opiteck. Semi-automated sample Preparation for Plasma Proteomics. J. Assoc. Laboratory Automation,. 2004;9:238–249.  

Hulmer J D. Betha K. Ho S.-P. Huang D. Ricci G. Opiteck S.A. Hefta. An Investigation of Plasma Collection, Stabilization, and Storage Procedures for Proteomic Analysis of Clinical Samples. Clinical Proteomics. 2004;1:17–32.  

2003


Gao J, Opiteck GJ, Friedrichs MS, Dongre AR, Hefta SA. Changes in the protein expression of yeast as a function of carbon source. J Proteome Res. 2003;2(6):643–649.  http://www.ncbi.nlm.nih.gov/pubmed/14692458

Kline TR, Pang J, Hefta SA, Opiteck GJ, Kiefer SE, Scheffler JE. A high-yield method to extract peptides from rat brain tissue. Anal Biochem. 2003;315(2):183–188.  http://www.ncbi.nlm.nih.gov/pubmed/12689828

2002


Pang J N. Ginanni A.R. Dongr. Biomarker Discovery in Urine by Proteomics. J. Proteome Res.. 2002;12:183–188.  

2001


Dongre AR G. Opiteck W.L. Cosand and S.A. Hefta. Proteomics in the Post-Genome Age. Biopolymers Pept Sci. 2001;60:206–211.  

2000


Dongre AR and SAH. Proteomics from a Drug Discovery Persepective. Drug Discovery World. 2000.  

1998


Tobi M E. Darmon P. Rozen M. Baratz M. Lundqvist K. Oberg P. Thomas J. Esteban S. Hefta R. Paxon J. Shively. Shared tumor antigens in colorectal carcinoma and neuroendocrine tumors. Cancer Detection and Prevention. 1998;22:147–152.  

1996


Klein ML, McGhee SA, Baranian J, Stevens L, Hefta SA. Role of nonspecific cross-reacting antigen, a CD66 cluster antigen, in activation of human granulocytes. Infect Immun. 1996;64(11):4574–4579.  http://www.ncbi.nlm.nih.gov/pubmed/8890209

1995


Davis MT, Lee TD, Ronk M, Hefta SA. Microscale immobilized protease reactor columns for peptide mapping by liquid chromatography/mass spectral analyses. Anal Biochem. 1995;224(1):235–244.  http://www.ncbi.nlm.nih.gov/pubmed/7710077

Davis MT, Stahl DC, Hefta SA, Lee TD. A microscale electrospray interface for on-line, capillary liquid chromatography/tandem mass spectrometry of complex peptide mixtures. Anal Chem. 1995;67(24):4549–4556.  http://www.ncbi.nlm.nih.gov/pubmed/8633788

1994


Pessolani MC, Smith DR, Rivoire B, et al. Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae. J Exp Med. 1994;180(1):319–327.  http://www.ncbi.nlm.nih.gov/pubmed/8006590

Rivoire B, Pessolani MC, Bozic CM, et al. Chemical definition, cloning, and expression of the major protein of the leprosy bacillus. Infect Immun. 1994;62(6):2417–2425.  http://www.ncbi.nlm.nih.gov/pubmed/7910593

1993


Enghild JJ, Salvesen G, Thogersen IB, Valnickova Z, Pizzo SV, Hefta SA. Presence of the protein-glycosaminoglycan-protein covalent cross-link in the inter-alpha-inhibitor-related proteinase inhibitor heavy chain 2/bikunin. J Biol Chem. 1993;268(12):8711–8716.  http://www.ncbi.nlm.nih.gov/pubmed/7682553

Kasuya J, Paz IB, Maddux BA, Goldfine ID, Hefta SA, Fujita-Yamaguchi Y. Characterization of human placental insulin-like growth factor-I/insulin hybrid receptors by protein microsequencing and purification. Biochemistry. 1993;32(49):13531–13536.  http://www.ncbi.nlm.nih.gov/pubmed/8257688

Mahrenholz AM, Yeh CH, Shively JE, Hefta SA. Microsequence and mass spectral analysis of nonspecific cross-reacting antigen 160, a CD15-positive neutrophil membrane glycoprotein. Demonstration of identity with biliary glycoprotein. J Biol Chem. 1993;268(18):13015–13018.  http://www.ncbi.nlm.nih.gov/pubmed/8099907

Sauter SL, Rutherfurd SM, Wagener C, Shively JE, Hefta SA. Identification of the specific oligosaccharide sites recognized by type 1 fimbriae from Escherichia coli on nonspecific cross-reacting antigen, a CD66 cluster granulocyte glycoprotein. J Biol Chem. 1993;268(21):15510–15516.  http://www.ncbi.nlm.nih.gov/pubmed/8101840

1992


Hefta L F. Chen M. Ronk S. Sauter V. Sarin S. Oikawa H. Nakazato S.A. Hefta J. Shively. Expression of carcinoembryonic antigen and its predicted immunoglobulin-like domains in HeLa cells for epitope analysis. Cancer Research. 1992;52:5647–5655.  

Lee BY, Hefta SA, Brennan PJ. Characterization of the major membrane protein of virulent Mycobacterium tuberculosis. Infect Immun. 1992;60(5):2066–2074.  http://www.ncbi.nlm.nih.gov/pubmed/1563797

1991


Enghild JJ, Salvesen G, Hefta SA, Thogersen IB, Rutherfurd S, Pizzo SV. Chondroitin 4-sulfate covalently cross-links the chains of the human blood protein pre-alpha-inhibitor. J Biol Chem. 1991;266(2):747–751.  http://www.ncbi.nlm.nih.gov/pubmed/1898736

Leusch HG, Drzeniek Z, Hefta SA, Markos-Pusztai Z, Wagener C. The putative role of members of the CEA-gene family (CEA, NCA an BGP) as ligands for the bacterial colonization of different human epithelial tissues. Zentralbl Bakteriol. 1991;275(1):118–122.  http://www.ncbi.nlm.nih.gov/pubmed/1718301

Mahrenholz AM, Hefta SA, Mansour TE. Phosphofructokinase from Fasciola hepatica: sequence of the cAMP-dependent protein kinase phosphorylation site. Arch Biochem Biophys. 1991;288(2):463–467.  http://www.ncbi.nlm.nih.gov/pubmed/1832841

Mooser G, Hefta SA, Paxton RJ, Shively JE, Lee TD. Isolation and sequence of an active-site peptide containing a catalytic aspartic acid from two Streptococcus sobrinus alpha-glucosyltransferases. J Biol Chem. 1991;266(14):8916–8922.  http://www.ncbi.nlm.nih.gov/pubmed/1827439

Sauter SL, Rutherfurd SM, Wagener C, Shively JE, Hefta SA. Binding of nonspecific cross-reacting antigen, a granulocyte membrane glycoprotein, to Escherichia coli expressing type 1 fimbriae. Infect Immun. 1991;59(7):2485–2493.  http://www.ncbi.nlm.nih.gov/pubmed/1675630

1990


Hefta SA, Paxton RJ, Shively JE. Sequence and glycosylation site identity of two distinct glycoforms of nonspecific cross-reacting antigen as demonstrated by sequence analysis and fast atom bombardment mass spectrometry. J Biol Chem. 1990;265(15):8618–8626.  http://www.ncbi.nlm.nih.gov/pubmed/2341397

Leusch HG, Hefta SA, Drzeniek Z, Hummel K, Markos-Pusztai Z, Wagener C. Escherichia coli of human origin binds to carcinoembryonic antigen (CEA) and non-specific crossreacting antigen (NCA). FEBS Lett. 1990;261(2):405–409.  http://www.ncbi.nlm.nih.gov/pubmed/2178978

Wong C, Hefta SA, Paxton RJ, Shively JE, Mooser G. Size and subdomain architecture of the glucan-binding domain of sucrose:3-alpha-D-glucosyltransferase from Streptococcus sobrinus. Infect Immun. 1990;58(7):2165–2170.  http://www.ncbi.nlm.nih.gov/pubmed/2142138

1988


Hefta SA, Hefta LJ, Lee TD, Paxton RJ, Shively JE. Carcinoembryonic antigen is anchored to membranes by covalent attachment to a glycosylphosphatidylinositol moiety: identification of the ethanolamine linkage site. Proc Natl Acad Sci U S A. 1988;85(13):4648–4652.  http://www.ncbi.nlm.nih.gov/pubmed/3387431

Hefta SA, Lyle SB, Busch MR, Harris DE, Matthew JB, Gurd FR. Site-specific semisynthetic variant of human hemoglobin. Proc Natl Acad Sci U S A. 1988;85(3):709–713.  http://www.ncbi.nlm.nih.gov/pubmed/3422453

Hinoda Y, Neumaier M, Hefta SA, et al. Molecular cloning of a cDNA coding biliary glycoprotein I: primary structure of a glycoprotein immunologically crossreactive with carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1988;85(18):6959–6963.  http://www.ncbi.nlm.nih.gov/pubmed/2457922